Effect of ultrasonic-assisted extraction method on the oil yield and fatty acid profile of Thraustochytrium multirudimentale and Rhodotorula mucilaginosa

Abstract This study aims to examine the effect of ultrasonic-assisted extraction method on yield oil and profile fatty acid from microalga yeast. The cultivated was identified genetically as Thraustochytrium multirudimentale MAST-1 yeast Rhodotorula mucilaginosa KAST-1. oils were extracted dried biomass by solvents using modified Bligh Dyer (BD) method. Before extraction, material underwent ultrasonication, water bath immersion, centrifugation for separation solvent. Extracted further analyzed compositions Gas Chromatography-Mass Spectrometry (GC-MS). An increase in length ultrasonication yielded a higher amount oil. cell walls disruption 60 minutes produced highest yield. BD’s best R. KAST-1 with 46.3% oil, while T. gave only 20.6% acids palmitic, stearic, oleic, elaidic pentadecylic acid, palmitoleic, pentadecylic, margaric, linoleic, elaidic, stearic erucic acid. Linoleic one polyunsaturated (PUFA) detected